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M94A2761.TXT
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1994-10-25
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Document 2761
DOCN M94A2761
TI Saliva-based HIV testing in Thailand.
DT 9412
AU Frerichs RR; Silarug N; Eskes N; Pagcharoenpol P; Rodklai A;
Thangsupachai S; Wongba C; UCLA, Department of Epidemiology, University
of California at Los; Angeles 90024-1772.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):230 (abstract no. PB0349). Unique
Identifier : AIDSLINE ICA10/94369814
AB OBJECTIVE: To determine if saliva could serve as a non-invasive
alternative to serum for testing of HIV antibodies in the on-going
sentinel surveillance program in Thailand. METHODS: Serum and saliva
specimens were gathered anonymously from 1,955 persons in four of 73
sentinel sites of the national surveillance program in Thailand. Saliva
was gathered with the Omni-Sal collection device (SDS) and analyzed with
the GACELISA (Murex). Serum served as the gold standard and was analyzed
with an ELISA test for screening and the WB for confirmation. Linkage
between serum and saliva was blind to the laboratory. Also analyzed
blind was a set of HIV+ and HIV- quality assurance samples for both
serum and saliva. RESULTS: The sensitivity of GACELISA with saliva was
98.0% in the field (298 HIV+), 100% after correction of errors (300
HIV+), and 100% among the quality assurance samples (95 HIV+). The
specificity of GACELISA was 99.4% in the field (1,653 HIV-), 99.6% after
correction of errors (1,654 HIV-), and 100% among the quality assurance
samples (96 HIV-). CONCLUSION: Our findings in the largest study of its
kind ever done, demonstrate that saliva is comparable to serum for
assessment of HIV antibodies in a surveillance program. The next step is
to develop a simple, inexpensive device for anonymous home screening.
DE Comparative Study Enzyme-Linked Immunosorbent Assay Evaluation Studies
Human HIV Antibodies/*ANALYSIS/BLOOD HIV Infections/*EPIDEMIOLOGY
Mass Screening/*METHODS *Population Surveillance Saliva/*IMMUNOLOGY
Salivary Proteins/*ANALYSIS Sensitivity and Specificity Single-Blind
Method Thailand/EPIDEMIOLOGY MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).